Highly sensitive quantitation of pesticides in fruit samples by modeling four-way data gathered with high-performance liquid chromatography with fluorescence excitation?emission detection

MILAGROS MONTEMURRO; LICARION PINTO; GERMANO VÉRAS; ADRIANO DE ARAÚJO GOMES; MARÍA J. CULZONI; MÁRIO C. UGULINO DE ARAÚJO; HÉCTOR C. GOICOECHEA

TALANTA

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2016

0039-9140

A study regarding the acquisition and analyticalutilization of four-way data acquired by monitoring excitation?emission fluorescencematrices at different elution time points in a fast HPLC procedure is presented.The data were modeled with three well-known algorithms: PARAFAC, U-PLS/RTL andMCR-ALS, the latter conveniently adapted to model third-order data. Thesecond-order advantage was exploited when analyzing samples containinguncalibrated components. The best results were furnished with the algorithm U-PLS/RTL.This fact is indicative of both no peak time shifts occurrence among samples andhigh colinearity among spectra. Besides, this latent-variable structuredalgorithm is capable of better handle the need of achieving high sensitivityfor the analysis of one of the analytes. In addition, a significant enhancementin both predictions and analytical figures of merit was observed for carbendazim, thiabendazole, fuberidazole, carbofuran, carbaryl and 1-naphtol, when going from second- to third-order data. LODsobtained were ranged between 0.02 and 2.4 mg L?1.