Extracting DNA of nematodes communities from Argentine Pampas agricultural soils

MONDINO EA; COVACEVICH F; STUDDERT, GA; PIMENTEL JP; BERBARA RLL

ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS.

ACAD BRASILEIRA DE CIENCIAS

Lugar: Rio de Janeiro; Año: 2015 vol. 87 p. 691 - 697

0001-3765

We examined four strategies (Tris/EDTA, sodium dodecyl sulfate, Chelex 100 resin and cetyltrimethylammonium bromide -CTAB-) for extracting nucleic acid (DNA) from communities of nematodes. Nematodes were isolated from an agricultural area under different management of long-term crop rotation experiment from Argentina during three seasons. After DNA extraction, Polymerase Chain Reaction-amplifications were performed and considered as indicators of successful DNA extraction. The CTAB combined with proteinase K and phenol-chloroform-isoamyl alcohol was the unique successful method because positive amplifications were obtained by using both eukaryotic and nematode specific primers. This work could contribute to biodiversity studies of nematodes on agroecosystems.