Chemometrics–assisted UV–spectroscopic strategies for the determination of theophylline in syrups.

MARÍA J. CULZONI; MARÍA M. DE ZAN; JUAN CARLOS ROBLES; VÍCTOR E. MANTOVANI; HÉCTOR C. GOICOECHEA

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Año: 2005 vol. 39 p. 1068 - 1074

0731-7085

AbstractTwo spectrophotometric methods, assisted by chemometric tools, were developed for the determination of theophylline in syrups: derivativespectroscopy (DS) and partial least-squares regression (PLS), the latter using both artificial and natural calibration sets. HPLC technique wasemployed to apply a reference method in order to achieve a complete assessment of performance. Calibrations presented excellent analyticalfigures of merits (i.e. LOD ranged from 0.03 to 0.4 mg L−1 and analytical sensitivity ranged from 1.7 to 8.3 Lmg−1). The intermediate precisionpresented pooled coefficients of variation ranged between 0.54 and 1.08%. Accuracy was studied trough an elliptical joint confidence regiontest (EJCR) comparing the obtained values when analysing spiked real samples by the HPLC and the studied methodologies. Both studiedmethods can be considered acceptable for the pharmaceutical quality assurance of theophylline in syrup samples.