Heavy metal toxicity in Exosphaeroma gigas (Crustacea, Isopoda) from the coastal zone of Beagle Channel

GIARRATANO; COMOGLIO; AMIN

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

Elsevier

Año: 2007 vol. 68 p. 451 - 462

0147-6513

Acute toxicity of copper, cadmium, and zinc on isopod Exosphaeroma gigas was evaluated at 20% and 30% salinity. Six concentrations were assayed to estimate effective concentration of the toxicant that affects 50% of tested animal (EC50), while physiological responses and bioaccumulation were determined at 0.42 and 1.95 mgL1 of each metal. The following toxicity orders were obtained: CdXCu4Zn at 20% salinity and Cu4ZnXCd at 30% salinity. Copper treatments showed a reduction in oxygen consumption at 30% salinity, while an opposite trend was observed at 20% salinity. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Copper treatments showed a reduction in oxygen consumption at 30% salinity, while an opposite trend was observed at 20% salinity. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. obtained: CdXCu4Zn at 20% salinity and Cu4ZnXCd at 30% salinity. Copper treatments showed a reduction in oxygen consumption at 30% salinity, while an opposite trend was observed at 20% salinity. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Copper treatments showed a reduction in oxygen consumption at 30% salinity, while an opposite trend was observed at 20% salinity. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. concentrations were assayed to estimate effective concentration of the toxicant that affects 50% of tested animal (EC50), while physiological responses and bioaccumulation were determined at 0.42 and 1.95 mgL1 of each metal. The following toxicity orders were obtained: CdXCu4Zn at 20% salinity and Cu4ZnXCd at 30% salinity. Copper treatments showed a reduction in oxygen consumption at 30% salinity, while an opposite trend was observed at 20% salinity. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Copper treatments showed a reduction in oxygen consumption at 30% salinity, while an opposite trend was observed at 20% salinity. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. obtained: CdXCu4Zn at 20% salinity and Cu4ZnXCd at 30% salinity. Copper treatments showed a reduction in oxygen consumption at 30% salinity, while an opposite trend was observed at 20% salinity. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Copper treatments showed a reduction in oxygen consumption at 30% salinity, while an opposite trend was observed at 20% salinity. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Exosphaeroma gigas was evaluated at 20% and 30% salinity. Six concentrations were assayed to estimate effective concentration of the toxicant that affects 50% of tested animal (EC50), while physiological responses and bioaccumulation were determined at 0.42 and 1.95 mgL1 of each metal. The following toxicity orders were obtained: CdXCu4Zn at 20% salinity and Cu4ZnXCd at 30% salinity. Copper treatments showed a reduction in oxygen consumption at 30% salinity, while an opposite trend was observed at 20% salinity. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Copper treatments showed a reduction in oxygen consumption at 30% salinity, while an opposite trend was observed at 20% salinity. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. obtained: CdXCu4Zn at 20% salinity and Cu4ZnXCd at 30% salinity. Copper treatments showed a reduction in oxygen consumption at 30% salinity, while an opposite trend was observed at 20% salinity. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Copper treatments showed a reduction in oxygen consumption at 30% salinity, while an opposite trend was observed at 20% salinity. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. 1 of each metal. The following toxicity orders were obtained: CdXCu4Zn at 20% salinity and Cu4ZnXCd at 30% salinity. Copper treatments showed a reduction in oxygen consumption at 30% salinity, while an opposite trend was observed at 20% salinity. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Copper treatments showed a reduction in oxygen consumption at 30% salinity, while an opposite trend was observed at 20% salinity. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.4220% mg L1. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations. Ammonia excretion was generally higher in treatments than control at 20% salinity. At 30% salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomi