Ganglioside GM1-binding peptides as adjuvants of antigens inoculated by the intranasal route

ALEJANDRO D. MONTANER,; ANALIA DE NICHILO,; FERNANDA ELIAS,; JUAN M. RODRIGUEZ,; JUAN M. FLÓ,; RICARDO A. LÓPEZ,; JORGE ZORZOPULOS,; RONALD FRANK

VACCINE

Elsevier

Año: 2006 vol. 24 p. 1889 - 1896

Forty-five GM1-binding peptides were identified using phage-displayed peptides libraries of random peptides. Most have a motif containing a hydrophobic amino acid followed by a serine (S). Based on a GM1-binding assays, two of these GM1-binding peptides (named 15 and 40) were chosen to investigate its immunostimulatory properties when chemically coupled to antigens. Mice intra-nasally (i.n.) vaccinated with some of these complexes developed a better local and systemic antibody response than mice i.n. vaccinated with the respective uncoupled antigens. The efficiency of the complex GM1-binding peptide-antigen strongly depends on the composition and structure of both of the components of the complex.