Trans-sialidase inhibition assay (TIA) detects Trypanosoma cruzi infection in different wild mammal species

SARTOR P.; CEBALLOS L.A.; OROZCO M; CARDINAL M.V.; GURTLER R; LEGUIZAMON MS

VECTOR-BORNE AND ZOONOTIC DISEASES

MARY ANN LIEBERT INC

Lugar: New York; Año: 2012

1530-3667

The detection of Trypanosoma cruzi infection in mammals is crucial for understanding the eco-epidemiological role of the different species involved in parasite transmission cycles. Xenodiagnosis (XD) and hemoculture (HC) are routinely used to detect T. cruzi in wild mammals. Serological methods are much more limited because they require the use of specific antibodies to immunoglobulins of each mammalian species susceptible to T. cruzi. In this study we detected T. cruzi infection by trans-sialidase inhibition assay (TIA). TIA is based on the antibody neutralization of a recombinant trans-sialidase that avoids the use of anti-immunoglobulins. Trans-sialidase activity is not detected in the co-endemic protozoan parasites Leishmania spp and T. rangeli. In the current study serum samples from 158 individuals of nine wild mammalian species, previously tested by XD, were evaluated by TIA. They were collected from two endemic areas in northern Argentina. The overall TIA vs XD co-reactivity was 98.7% (156/158). All 18 samples from XD-positive mammals were TIA-positive (co-positivity, 100%) and co-negativity was 98.5% (138/140). Two XD-negative samples from a marsupial (Didelphis albiventris) and an edentate (Dasypus novemcinctus) were detected by TIA. TIA could be used as a novel tool for serological detection of Trypanosoma cruzi in a wide variety of sylvatic reservoir hosts.