High-performance liquid chromatography with fast-scanning fluorescence detection and multivariate curve resolution for the effcient determination of galantamine and its main metabolites in serum

MARÍA J. CULZONI; RICARDO Q. AUCELIO; GRACIELA M. ESCANDAR

ANALYTICA CHIMICA ACTA

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2012 vol. 740 p. 27 - 35

0003-2670

Based on green analytical chemistry principles, an efficient approach was applied for the simultaneous determination of galantamine, a widely used cholinesterase inhibitor for the treatment of Alzheimer?s disease, and its major metabolites in serum samples. After a simple serum deproteinization step, second- order data were rapidly obtained (less than 6 min) with a chromatographic system operating in theisocratic regime using ammonium acetate/acetonitrile (94:6) as mobile phase. Detection was made witha fast-scanning spectrofluorimeter, which allowed the efficient collection of data to obtain matrices offluorescence intensity as a function of retention time and emission wavelength. Successful resolution was achieved in the presence of matrix interferences in serum samples using multivariate curve resolution-alternating least-squares (MCR-ALS). The developed approach allows the quantification of the analytesat levels found in treated patients, without the need of applying either preconcentration or extraction steps. Limits of detection in the range between 8 and 11 ng mL−1, relative prediction errors from 7 to 12% and coefficients of variation from 4 to 7% were achieved.