A micellar-enhanced fluorescence photoinduced four-way calibration method for the determination of multiclass pesticides in lemon juice

MARINA ANTONIO; MIRTA R. ALCARÁZ; RUBÉN DARÍO FALCONE; CULZONI MARÍA JULIA

Analytica Chimica Acta

Elsevier B.V.

Año: 2023 vol. 1279

In this work, a four-way multivariate calibration method for the simultaneous determination of four pesticides -carbendazim (CBZ), thiabendazole (TBZ), pirimiphos-methyl (PMM), and clothianidin (CLT) - in lemon juice ispresented. Third-order data were acquired by registering the photoinduced fluorescence of the analytes asexcitation-emission matrices at different times of UV-light irradiation, in the presence of organized media (directmicelles) as fluorescence enhancers. The optimal experimental conditions (pH 11.5 and 32 mmol L􀀀 1 hexadecyltrimethylammoniumchloride surfactant) were determined through a central composite design using theresponse surface methodology. The analytes were individually calibrated, except for TBZ and CBZ due to theinner filter effect of TBZ on CBZ. Test samples containing all analytes and imidacloprid (as potential interference)were analysed. PARAFAC was utilized to evaluate both the trilinearity and quadrilinearity of the third-order dataand four-way arrays, respectively. PMM was successfully determined with quadrilinear PARAFAC decomposition,whereas CLT, TBZ, and CBZ were satisfactorily modelled using U-PLS/RTL due to the loss of quadrilinearitycaused by different phenomena. The profitable applicability of the analytical method in the CBZ, TBZ, PMM, andCLT determination in lemon juice samples was demonstrated, achieving limits of detection below the maximumresidue levels reported by the European Commission, and mean recoveries at 90 ± 5%.