Enzymatic hydrolysis as a valorization strategy of bovine lungs: Optimization of process variables and study of antioxidant capacity

MARTINEZ, F. G.; AMBROSI, V. A.; ROCHA, G.; SANCHO, A. M.; SZERMAN, N.

JSFA reports

Wiley

Lugar: Nueva York; Año: 2023 vol. 3 p. 161 - 169

2573-5098

A response surface methodology study was carried out using a central compos-ite design in which factors were temperature (43.2–76.8C), enzyme/substrate ratio [ES:0.65–4.85% (w/w)] using Alcalase 2.4 L/Flavourzyme (1:1) and pH (5.8–9.2). The antioxi-dant capacity of the obtained BLH (bovine lung hydrolysates) was evaluated by theABTS, DPPH, and FRAP methods. The maximum antioxidant capacity was achieved at53.C, ES ratio of 2.3% (w/w), and pH of 8.2. Under optimal hydrolysis conditions, theantioxidant capacity increased rapidly from the beginning of the reaction to 30 min,reaching a maximum at 120 min; then, it decreased until the end (180 min). The degreeof hydrolysis increased as hydrolysis occurred up to a maximum of 45%. Molecularweight distribution of BLH evaluated by Tricine-SDS-PAGE and SEC-FPLC showed thatbovine lung proteins hydrolyzed for 120 min presented low molecular weight peptides(<5 kDa), which are absent in the non-hydrolyzed sample.