INTEC   05402
INSTITUTO DE DESARROLLO TECNOLOGICO PARA LA INDUSTRIA QUIMICA
Unidad Ejecutora - UE
artículos
Título:
Study of milk/k-carragenan mixtures by atomic force microscopy
Autor/es:
FERRON, J.; PASSEGGI, M.; OLIVARES, M. L.; ZORRILLA, SE; AMELIA CATALINA RUBIOLO
Revista:
Food Hydrocolloids
Editorial:
Elsevier Ltd
Referencias:
Año: 2010 vol. 24 p. 766 - 782
Resumen:
Structural characteristics of milk components and milk/k-carrageenan mixtures were studied by
atomic force microscopy (AFM). Samples were adsorbed on highly ordered pyrolytic graphite (HOPG)
and mica substrates. Sample attachment by physisorption and imaging in air by AFM resulted in
a very promising technique to investigate structures and interactions between biological macromolecules
under near-native conditions. Differences in adsorption of casein micelles on HOPG and mica
surfaces were observed. Casein micelles were adsorbed satisfactorily on HOPG while poor adsorption
was obtained on mica surface. Topographical images of casein micelles adsorbed on HOPG denoted
pseudo-spherical structures forming aggregates which were polydisperse in size and shape. In the case
of milk/k-carrageenan mixtures, it was observed that as k-carrageenan concentration increased, the
amount of casein micelles attached on HOPG surface decreased. An opposite behavior was observed
when samples were adsorbed on mica. Hydrophobic and hydrophilic interactions between samples
and substrates helped in improving the understanding of the interactions between the macromolecules
studied. Our findings agreed with previous studies suggesting a surface level interaction
between casein micelles and k-carrageenan, the structures observed being dependent on polysaccharide
concentration. It can also be concluded that mica may be a suitable substrate for imaging
milk/k-carrageenan mixtures.k-carrageenan mixtures were studied by
atomic force microscopy (AFM). Samples were adsorbed on highly ordered pyrolytic graphite (HOPG)
and mica substrates. Sample attachment by physisorption and imaging in air by AFM resulted in
a very promising technique to investigate structures and interactions between biological macromolecules
under near-native conditions. Differences in adsorption of casein micelles on HOPG and mica
surfaces were observed. Casein micelles were adsorbed satisfactorily on HOPG while poor adsorption
was obtained on mica surface. Topographical images of casein micelles adsorbed on HOPG denoted
pseudo-spherical structures forming aggregates which were polydisperse in size and shape. In the case
of milk/k-carrageenan mixtures, it was observed that as k-carrageenan concentration increased, the
amount of casein micelles attached on HOPG surface decreased. An opposite behavior was observed
when samples were adsorbed on mica. Hydrophobic and hydrophilic interactions between samples
and substrates helped in improving the understanding of the interactions between the macromolecules
studied. Our findings agreed with previous studies suggesting a surface level interaction
between casein micelles and k-carrageenan, the structures observed being dependent on polysaccharide
concentration. It can also be concluded that mica may be a suitable substrate for imaging
milk/k-carrageenan mixtures.near-native conditions. Differences in adsorption of casein micelles on HOPG and mica
surfaces were observed. Casein micelles were adsorbed satisfactorily on HOPG while poor adsorption
was obtained on mica surface. Topographical images of casein micelles adsorbed on HOPG denoted
pseudo-spherical structures forming aggregates which were polydisperse in size and shape. In the case
of milk/k-carrageenan mixtures, it was observed that as k-carrageenan concentration increased, the
amount of casein micelles attached on HOPG surface decreased. An opposite behavior was observed
when samples were adsorbed on mica. Hydrophobic and hydrophilic interactions between samples
and substrates helped in improving the understanding of the interactions between the macromolecules
studied. Our findings agreed with previous studies suggesting a surface level interaction
between casein micelles and k-carrageenan, the structures observed being dependent on polysaccharide
concentration. It can also be concluded that mica may be a suitable substrate for imaging
milk/k-carrageenan mixtures.k-carrageenan mixtures, it was observed that as k-carrageenan concentration increased, the
amount of casein micelles attached on HOPG surface decreased. An opposite behavior was observed
when samples were adsorbed on mica. Hydrophobic and hydrophilic interactions between samples
and substrates helped in improving the understanding of the interactions between the macromolecules
studied. Our findings agreed with previous studies suggesting a surface level interaction
between casein micelles and k-carrageenan, the structures observed being dependent on polysaccharide
concentration. It can also be concluded that mica may be a suitable substrate for imaging
milk/k-carrageenan mixtures.findings agreed with previous studies suggesting a surface level interaction
between casein micelles and k-carrageenan, the structures observed being dependent on polysaccharide
concentration. It can also be concluded that mica may be a suitable substrate for imaging
milk/k-carrageenan mixtures.k-carrageenan, the structures observed being dependent on polysaccharide
concentration. It can also be concluded that mica may be a suitable substrate for imaging
milk/k-carrageenan mixtures.k-carrageenan mixtures.