INVESTIGADORES
MENDOZA Lucia Margarita
artículos
Título:
Phenotypic surface properties (aggregation, adhesion and biofilm formation) and presence of related genes in beneficial vaginal lactobacilli
Autor/es:
M. CECILIA LECCESE TERRAF; LUCÍA M. MENDOZA; M. SILVINA JUAREZ TOMAS; CLARA SILVA; M. ELENA FATIMA NADER-MACÍAS
Revista:
JOURNAL OF APPLIED MICROBIOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2014 vol. 117 p. 1761 - 1772
ISSN:
1364-5072
Resumen:
Aims:
To evaluate the phenotypic expression of auto-aggregation, adhesion to mucin
and biofilm formation of lactobacilli isolated from human vagina and the
presence of related genes. Methods and Results: Seven different strains of
three Lactobacillus species (Lactobacillus gasseri, Lactobacillus rhamnosus and
Lactobacillus reuteri) were evaluated. The auto-aggregation property was
determined by spectrophotometric assay and flow cytometry. Adhesion and biofilm
formation were assayed by crystal violet staining. The presence of the genes
encoding sortases, pilin subunits and surface proteins was evaluated by
polymerase chain reactions. The two Lact. reuteri strains assayed showed high
auto-aggregation, adhesion to mucin and biofilm formation ability. In these
strains, the genes encoding three adhesion proteins were identified. In Lact.
rhamnosus CRL (Centro de Referencia para Lactobacilos Culture Collection) 1332,
pilus-encoding genes were detected. In all Lact. rhamnosus strains assayed, two
genes encoding for other surface proteins related to adhesion and biofilm
formation were detected. Conclusions: The vaginal lactobacilli assayed
exhibited phenotypic and genetic characteristics that were specific for each
strain. Significance and Impact of the Study: This is the first study on auto-aggregation,
adhesion and biofilm formation of vaginal Lactobacillus strains by phenotypic
and genetic assays.