Removal of Rab11 endosomes affects spines morphology and dendritic arbor development

SEBASTIÁN OMAR SIRI; QUASSOLLO, GONZALO; CONDE, CECILIA

Mar del Plata

Congreso; Sociedad Argentina de investigacion en Neurociencia 2017; 2017

SAN

The neuronal dendritic arbor development requires a proper spatiotemporal distribution of the elements of the secretory and endocytic pathways, as wellas a dynamic and regulated communication between them. Accordingly, our workhas focused on characterizing the role of the recycling endosome (RE) duringthe development of the dendritic arbor in primary embryonic hippocampalneurons. We first determined the endogenous localization of the RE at differenttimes in culture from day 1 to 21. In the first 24 hours the RE shows ajuxtanuclear distribution and on the process tips. After 24 hours the RE,changes its localization to a more broad distribution throughout the neuritesuntil the day 14 where it mainly concentrate on the processes tips.Upon using a shRNA-Rab11 we observed morphological changes of the normaldevelopment of dendritic arborization. In addition, we observed a shortening ofthe main neurite and a concomitant increase in number of dendritic branches.Moreover, suppression of Rab11 resulting a significant change in distributionof two membrane receptors protein (TfR and GluRI). Taken together, ourexperimental results suggest that the temporal and spatial localization ofRab11 endosomes have a distribution dependent on the stage of neuronaldevelopment. This localization could be a possible requirement for theelongation of dendritic processes.