A specific immunological method to detect and quantify bacterial2-substituted (1,3)-B-d-glucan

MARÍA LAURA WERNING,; ADRIÁN PÉREZ RAMOS; PILAR FERNÁNDEZ DE PALENCIA; MARI LUZ MOHEDANO; MARÍA TERESA DUEÑAS; ALICIA PRIETO; PALOMA LÓPEZ

CARBOHYDRATE POLYMERS

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2014 vol. 113 p. 39 - 45

0144-8617

tExopolysaccharides synthesized by lactic acid bacteria have prebiotic properties and contribute to therheology and texture of fermented foods. Here, we have standardized an immunological method for thespecific detection of 2-substituted (1,3)-b-d-glucans. The method allows direct detection and quantifi-cation of this exopolysaccharide in culture supernatants containing other mono- and poly-saccharides.Moreover, it allows specific detection of the biomolecules synthesized in vitro in enzymatic reactions.Thus, this method allows the fast identification of producing bacteria, as well as biochemical characteri-zation of the glycosyltransferases responsible for their synthesis