Gut permeability and glucose absorption are affected at early stages of graft rejection in a small bowel transplant rat model

STRINGA, PABLO; ROMANIN, DAVID; LAUSADA, NATALIA; GOBI, RODRIGO PAPA; VECCHIO, LEANDRO; CABBANE, A; GONDOLESI, GABRIEL; RUMBO, MARTIN

New York

Congreso; XV INTERNATIONAL CONGRESS OF THE INTESTINAL REHABILITATION & TRANSPLANT ASSOCIATION (CIRTA 2017); 2017

Intestinal Rehabilitation and Transplant Association

Intestinal transplantation (IT) faces many challenges, among them, the necessity to early detect rejection processes. Rodent models of IT are used to provide evidence for intervention strategies as well as improve knowledge of small bowel transplant biology. The aim of our study was to determine the kinetics of small bowel rejection using a Heterotopic IT (HIT) model, with emphasis in the characterization of the process using different approaches, including the use of simple functional assays of absorptive and barrier function evaluation that could be eventually translated to the clinic.Allogeneic HIT was performed following standard procedure and rejection was monitored by clinical scoring and histopathology analysis. In order to evaluate graft absorptive function, a solution containing glucose (2 gr/Kg) and ovalbumin (OVA) (150 ug/dose) diluted in 1.5 ml of normal saline was administered by proximal ostomy 5 and 10 to 12 days after HIT. Blood samples were obtained for glucose and OVA determination at different times post-treatment.In allogeneic HIT recipients rejection kinetics was coincident to previously described, with mild rejection beginning by day 5 post-IT, becoming severe by day 10 post-IT (Figure 1). Interestingly, either at 5 or 10 to 12 post-IT days, glucose levels did not change from the basal value (101 +/- 21 mg/dL) in this group. In most of evaluated cases 5 days after HIT and in all cases evaluated at 10-12 post-IT days in allogeneic groups, OVA was detected in the recipient´s serum (24 +/-11 ug/mL, p