Effect of ischemic preconditioning, and tacrolimus pre-treatment as strategies to attenuate intestinal ischemia-reperfusion injury in a mouse model

STRINGA, P; LAUSADA, N; MACHUCA, M; ROMANIN, D; CABBANE, A; RUMBO, M; GONDOLESI, G

Washington DC

Simposio; XII International Small Bowel Transplant Symposium; 2011

Georgetown University - International Transplant Assosiation

background: among the organs, intestine is probably the most sensitive to ischemia reperfusion injury (IrI), independently of the cause. Intestinal IrI may alter the integrity of the mucosal enteric barrier, causing bacterial translocation, sepsis and triggering a systemic inflammatory response syndrome leading to multi organ failure and death. Different degrees of IrI occurred after intestinal transplantation, therefore to establish strategies to mitigate IrI is a major objective for basic and translational research in the field of today’s intestinal surgery. the aim of the present study was to evaluate the effect of ischemic preconditioning (IPC) and pre-treatment with tacrolimus (tC) in intestinal damage after immediate reperfusion. Material and Methods:36 adult male balb/c mice weighing 30 ± 3 grams were divided in 4 groups with 9 mice each: group C, (n=9) 40 min occlusion of superior mesenteric artery (oSMa) was applied follow by reperfusion without treatment; group tC: was pre-treated with intragastric administration of tC 3mg/kg 12 hs before oSMa. group IPC: a cycle of ten minutes of intestinal ischemia followed by ten minutes of reperfusion was performed before oaMS. finally a Sham group (SH) was included. thirty minutes post reperfusion; samples of distal jejunum were obtained from five animals in each group. Histological damage was evaluated using Park´s score. the remaining animals were followed for 24 hours to analyze survival. results:SH group showed normal Jejunum. Park’s value for IPC was 2.2 ± 0.8, tC 2.8 ± 0.4 and C 4.2 ± 0,8 (p