Probiotic yeast Kluyveromyces marxianus CIDCA 8154 shows anti-inflammatory and anti-oxidative stress properties in different in vivo models

ROMANIN, D; LLOPIS, S; GENOVÉS, S; MARTORELL, P; RAMÓN, D; GARROTE, G; RUMBO, M

Beneficial Microbes

Wageningen Academic Publishers

Lugar: Wageningen; Año: 2015

1876-2883

Inflammatorybowel diseases (IBDs) are complex affections with increasing incidenceworldwide. Multiple factors are involved in the development and maintenance ofthe symptoms including enhanced oxidative stress in intestinal mucosa. Theconventional therapeutic approaches for IBDs are based on the useanti-inflammatory drugs with important collateral effects and partial efficacy.In the present work we tested the anti-inflammatory capacity of Kluyveromyces marxianus CIDCA 8154 in different models. In vitro, we showed that thepretreatment of epithelial cells with the yeast reduce the levels ofintracellular reactive oxygen species. Furthermore, in a murine model ofTNBS-induced colitis, yeast-treated animals showed a reduced histopathologicalscore (p<0.05) and lower levels of circulating IL6 (p<0.05). The capacityto modulate oxidative stress in vivowas assessed using Caenorhabditis elegansmodel. The yeast was able to protect the nematodes from oxidative stress bymodulating the SKN-1 transcription factor trough the DAF-2 pathway. Theseresults indicate that Kluyveromycesmarxianus CIDCA 8154 could control the intestinal inflammation and cellularoxidative stress by mechanisms that differ to the ones used by other probiotics.This evidence is useful for the rational formulation of products containingmicroorganisms that trigger different anti-inflammatory pathways