INVESTIGADORES
SALINAS Maria Victoria
artículos
Título:
Synthesis, characterization, antitumoral and osteogenic activities of quercetin vanadyl (IV) complexes
Autor/es:
EVELINA G. FERRER; MARIA V. SALINAS; MARIA J. CORREA; LUCIANA NASO; DANIEL A. BARRIOS; SUSANA B. ETCHEVERRY; LUIS LEZAMA; TEÓFILO ROJO; PATRICIA A.M. WILLIAMS
Revista:
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
Editorial:
Springer
Referencias:
Lugar: Berlin, Alemania; Año: 2006 vol. 11 p. 791 - 801
ISSN:
0949-8257
Resumen:
The development of new vanadium derivatives
with organic ligands, which improve the beneficial
actions (insulin-mimetic, antitumoral) and decrease the
toxic effects, is of great interest. A good candidate for
the generation of a new vanadium compound is the
flavonoid quercetin because of its own anticarcinogenic
effect. The complex [VO(Quer)2EtOH]n (QuerVO)
has been synthesized and characterized by means of
different spectroscopic techniques (UV?vis, Fourier
transform IR, electron paramagnetic resonance) and
its magnetic and stability properties. The inhibitory
effect on bovine alkaline phosphatase (ALP) activity
has been tested for the free ligand, the complex as well
as for the vanadyl(IV) (comparative purposes). The
biological activity of the complex on the proliferation
of two osteoblast-like cells in culture, a normal one
(MC3T3E1) and a tumoral one (UMR106), has been
compared with that of the vanadyl(IV) cation and
quercetin. The differentiation osteoblast markers ALP
specific activity and collagen synthesis have been also
tested. In addition, the effect of QuerVO on the activation
of the extracellular regulated kinase (ERK)
pathway is reported. The bone antitumoral effect of
quercetin alone was established with the cell proliferation
assays (it inhibits the proliferation of the tumoral
cells and does not exert any effect on the normal osteoblasts).
Moreover, the complex exerts osteogenic
effects since it stimulates the type I collagen production
and is a weak inhibitory agent upon ALP activity.
Finally, QuerVO stimulated the ERK phosphorylation
in a dose?response manner and this activation seems to
be involved as one of the possible mechanisms for the
biological effects of the complex.