INVESTIGADORES
DE BRASI Carlos Daniel
artículos
Título:
Specific analysis of the intron 22 XbaI polymorphism of the human factor VIII gene using long-distance PCR.
Autor/es:
DE BRASI CD, BOWEN DJ, COLLINS PW, LARRIPA IB.
Revista:
BRITISH JOURNAL OF HAEMATOLOGY
Editorial:
Blackwell Science Ltd
Referencias:
Lugar: Oxford, UK; Año: 1999 vol. 107 p. 566 - 568
ISSN:
0007-1048
Resumen:
A rapid, non-radioactive, PCR-based method to genotype the XbaI restriction fragment length polymorphism of the human factor VIII gene is described. The method uses long-distance PCR followed by XbaI restriction digestion and agarose gel electrophoresis. The 6.6 kb amplification product includes a constant XbaI site, which provides a digestión control. The specificity of the method was challenged by a blind experiment with 16 genomic DNA samples previously genotyped by Southern blot analysis: a perfect correlation was obtained between genotypes determined using Southern blot and PCR.
