Lactobacillus rhamnosus RC007 intended for feed additive: Immune-stimulatory properties and ameliorating effects on TNBS-induced colitis

C. DOGI; A. DE MORENO DE LEBLANC; C. GRECO; L. CAVAGLIERI; L. CAVAGLIERI

Beneficial Microbes

Wageningen : Wageningen Academic Publishers

Año: 2016 vol. 7 p. 539 - 547

1876-2883

The aims of the present work was to evaluate in vivo the beneficial properties of L.  rhamnosus RC007, intended for animal feed, when administered to healthy and trinitrobenzene-sulfonic-acid (TNBS) colitis induced BALB/c mice. The administration of L. rhamnosus RC007 to healthy mice during 10 days increased the phagocytic activity of peritoneal macrophages and the number of IgA+ cells in the lamina  propria of the small intestine. Significant increases of MCP-1; IL-10 and TNFα concentrations, and in the ratio between anti- and pro-inflammatory cytokines (IL-10/TNFα) were observed in intestinal fluids after bacteria administration. In the inflammation model, less body weight loss, macroscopic and histological damages in the large intestine were accompanied by increased IL-10/TNFα ratio in the intestinal fluids of mice from L. rhamnosus-TNBS group when compared to TNBS group. Conclusions: In healthy host, the oral administration of L. rhamnosus RC007 kept the gut immune system stimulated allowing a faster response to noxious stimulus. Mice that received L. rhamnosus RC007 also decreased the severity of the intestinal inflammation.Significance and impact of the study: L. rhamnosus RC007 is a potential probiotic bacterium that can exert beneficial effect as supplement for animal feed, by improving the immune status in healthy host, and by providing therapeutic benefits to infected/inflamed animals.