CONICET | Buscador de Institutos y Recursos Humanos

Currently, organochlorine pesticides (OPs) are restricted or banned from agriculturaland public health areas. However, many of these compounds have been found in highconcentrations in contaminated sites, and therefore continue to pose environmental andhealth concerns. Thus, it is imperative to identify efficient microorganisms to removethem and to develop methods to remediate environments contaminated with OPs. In thisregard, the aim of this work was to evaluate the individual and simultaneous removal ofthree OPs; specifically lindane (LIN), γ-chlordane (CLD) and methoxychlor (MTX) (2mg L-1 of each pesticide alone or as constituent of a mixture), from liquid contaminatedsystems, employing a native and non-GMO Streptomyces strain. Toxicity test of thetreated systems against the cell line Caco-2, and the detection in the genome of the usedmicroorganism of sequences related with the pesticides catabolism are reported. Theresults demonstrated that, Streptomyces sp. A5 was able to remove individually 57.4%,100.0% and 6.5% of LIN, CLD and MTX, respectively. From the pesticide mixture,LIN and CLD were efficiently removed on 62.2% and 68.6% respectively by thestudied strain. On the other hand, we demonstrated that the liquid systems contaminatedwith the OPs mixture and bioremediated with Streptomyces sp. A5 showed lowercytotoxicity than their respective controls without inoculate. Moreover, molecularstudies revealed the presence in Streptomyces sp. A5 of putative lin genes with potentialdehydrochlorinase, haloalkane dehalogenase and NAD-dependent dehydrogenaseactivities. Therefore, the potential of Streptomyces sp. A5 to efficiently remediate OPsalone or in mixtures from liquid contaminated system, was demonstrated

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