KINETICS MODELING OF THE ACIDOLYSIS WITH IMMOBILIZED RHIZOMUCOR MIEHEI LIPASES FOR PRODUCTION OF STRUCTURED LIPIDS FROM SUNFLOWER OIL

CAMILA A. PALLA; MARÍA ELENA CARRÍN

BIOCHEMICAL ENGINEERING JOURNAL

ELSEVIER SCIENCE SA

Lugar: Amsterdam; Año: 2014 vol. 90 p. 184 - 194

1369-703X

Este trabajo ha sido ACEPTADO para su publicación el 10 de Junio de 2014-------Sunflower oil modification for production of semisolid fats was carried out via acidolysis using palmitic and stearic acids (P+St) and a novel biocatalyst from Rhizomucor miehei lipases. Its kinetic behavior was studied by employing three mathematical models proposed in the literature. Furthermore, a new model was proposed to describe not only the variation of triacylglycerols (TAG), diacylglycerols (DAG), and free fatty acids groups but also the acyl migration reaction occurrence. The effect of the reaction conditions on the kinetic and equilibrium parameters, as well as TAG and reaction intermediates profiles was analyzed. Increasing reaction temperature generated major changes in the overall composition of acylglycerols and gave rise to the highest composition of P+St in the obtained structured lipids (58%, 70 h, 60 °C). P+St incorporation was successfully adjusted by an empirical model and a lumped parameter model for all the studied reaction times, while the model based on a Ping Pong Bi Bi mechanism was only able to describe the kinetics behavior until 24 h. Experimental data were fit satisfactorily by the proposed model, showing that the increment in the disaturated TAG formation achieved by the increment in temperature was principally related to the favored DAG formation from triunsaturated TAG.