Polymeric And Strontium Composite Based Scaffold For Bone Tissue Engineering

LINO A; TORRES ML; MCCARTHY AD; FERNANDEZ JM

Workshop; II Workshop on Bio-degradable Polymers and Biocomposites and III Workshop BIOPURFIL, Bio-based Polyurethane Composites with Natural Fillers; 2015

Bone tissue engineering (BTE) uses different materials to design scaffolds that guide bone regeneration. In our group we have developed various polymers for application in BTE. One of these is a compatibilized blend of a polyester (poly-ε-caprolactone, PCL) and a polyfumarate (poly diisopropyl fumarate, PDIPF). This blend shows better osteocompatibility for cultured bone marrow progenitor cells (BMPC) than the respective homopolymers, in addition to no toxicity [1]. Strontium ranelate is a drug with dual anti-catabolic and anabolic action on bone metabolism, and is used for treatment of osteoporosis. We have previously loaded the compatibilized blend with 1% and 5% of Sr+2, and found that it does not release significant amounts of Sr+2 into the culture medium. However, the scaffold with 1% Sr+2 increased phosphatase alkaline activity (ALP) of BMPC cultures, while 5% Sr+2 decreased their ALP [2]. The objective of this study was to evaluate if the Sr+2-loaded polymer induces similar effects on BMPC type 1 collagen production, as well as its possible cytotoxicity (NO and LDH release).