Study of growth and differentiation of BMSC on synthetic polymeric matrices

M.S. MOLINUEVO; J.M. FERNÁNDEZ; M.S. CORTIZO; A.M. CORTIZO

Porto, Portugal

Congreso; Tissue Engineering and Regenerative Medicine International Society; 2008

TERMIS-EU

Resumen: <!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Bone tissue has the ability to repair minor injuries through remodeling. However, when the host source of osteoprogenitors is compromised at the defect site, one effective treatment may be cell-based therapy. These cells may be supported in a synthetic biodegradable scaffold to regenerate bone lesion. In this work we study poly-ester- and polyfumarate-scaffolds and their biocompatibility with bone marrow mesenchymal stem cells (BMSC). Surface properties and matrix porosity were evaluated by scanning electron microscopy (SEM), and hidrophilicity (water contact angle) of poly-e-caprolactone (PCL) and poly-diisopropyl-fumarate (PFIP) scaffolds obtained by solvent casting and lyophilization. PFIP showed a smooth surface while PCL presented some rugosities on its surface. Scaffolds obtained by lyophilization presented well distributed and homogeneous porosity compatible with bone microarchitechture. PCL presented higher hydrophylicity than PFIP. However, neither PCL nor PFIP were degraded after 21 days of incubation at 37ºC in phosphate saline buffer. Biocompatibility was evaluated by culturing BMSC on plastic (control) or polymeric matrices and incubated at 37ºC for different periods of time. Cell morphology, adhesion (1h), cell proliferation (24h) or osteoblastic differentiation (15 days) was evaluated. BMSC growing on the matrices developed a well defined stress fibers network without cytotoxicity signs. Cells attached and grew well on the scaffolds and expressed the osteoblastic marker alkaline phosphatase after 15 days in culture, at a similar rate that the cells growing in the control dishes. In conclusion, our results suggest that these polymeric scaffolds are suitable for bone tissue engineering purpose.