Biocompatibility study of polymeric matrices with application in Bone Tissue Engineering: Evaluation of angiogenic capacity

HURTADO CUBA, AG; TORRES ML; OBERTI TG; FERNANDEZ JM

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias 2022, SAIC, SAI y SAFIS; 2022

Bone Tissue Engineering (BTE) is an interdisciplinary science that uses materials to generate matrices that support cells and promote bone repair. Due to its key role in skeletal formation and repairing, we study the angiogenic capacity evaluating the proliferation of rat aortic smooth muscle cells (ASMC) and EA.hy926 endothelial cells on a family of hydrogels (HG). The HG is a polymer network of poly 2-hydroxyethylmethacrylate (pHEMA) cross-linked with ethylene glycol dimethylacrylate (E) with and without Alginate (Alg). The E concentration was modified (1, 0.5 and 0.25%) to analyzed this effect over final biomaterials properties. The angioinductive capacity of the bone marrow progenitor cells (BMPC) grown on the HG was evaluated using the culture media after 0 and 7d to analyze the migration of ASMC and EA.hy926 (cell monolayer wound method). We found that proliferation after 48h (MTT technique) of the ASMC on the HG was greater in pHEMA-Alg-1%E (125±2) and in pHEMA-alg-0.5%E (117±2) compared to pHEMA without alginate (100%±3%), but not in pHEMA-alg-0.25%E (99±3). After 48 h of culture, the proliferation of EA.hy926 cells on pHEMA-alg-1%E, 0.5%E and 0.25%E were: (141±2), (123±3) and (118±1 ), respectively, largest than pHEMA (100%±2%). We found an increase in the migration of ASMC when using the conditioned medium of the BMPC cells grown on pHEMA-Alg-1%E (211±4) and on pHEMA-alg-0.5%E (180±5) but not in pHEMA-alg-0.25%E (106±3) compared to pHEMA without alginate (100%±3%). Regarding the EA.hy926 cells, an increase in migration was observed when pHEMA-Alg-1%E (138±4) and pHEMA-alg-0.5%E (128± 2) was used but not pHEMA-alg-0.25%E (102±3) compared to pHEMA without alginate (100%±2%). Although we previously demonstrated that the 3 HG containing Alg could be good candidates for BTE, only those containing 1 and 0.5% E produce an increase in the angiogenic capacity necessary for bone repair.