INVESTIGADORES
PEREYRA Carina Maricel
congresos y reuniones científicas
Título:
In vitro inhibition of Aspergillus carbonarius growth and ochratoxin A production by beneficial Saccharomyces cerevisiae strains
Autor/es:
ARMANDO MR ; DOGI CA; POLONI V; PEREYRA CM; ALONSO V; LANDA F; DALCERO AM; CAVAGLIERI LR
Lugar:
Rhotterdam
Reunión:
Simposio; 7 th conference of the world mycotoxin forum and XIII IUPAC international symposium on mycotoxins and phicotoxins; 2012
Institución organizadora:
IUPAC
Resumen:
Mycotoxin
contamination is a serious problem in developing countries where climatic
conditions and agricultural and storage practices are conducive to fungal
growth and mycotoxin production. Ochratoxin A (OTA) is a mycotoxin with
nephrotoxic, teratogenic, immunosuppressive, and carcinogenic properties.
Aspergillus carbonarius seems to be the most important source of OTA because of
the high proportion of producing strains and high amounts produced. The
influence of Saccharomyces cerevisiae RC008 and RC016, previously selected for
their aflatoxin B1 binding ability and beneficial properties, against A.
carbonarius under different interacting environmental conditions was evaluated.
Studies on the lag phase, growth rate and OTA production were carried out under
different regimes of aw (0.95 and 0.99); pH (4 and 6); temperature (25 and
37°C) and oxygen availability (normal and reduced). Both yeast strains showed a
great antagonistic activity, decreasing growth rate compared to the control.
The RC016 strain showed the greatest inhibitory activity at all assayed
conditions. Aspergillus carbonarius was able to produce large amounts of OTA. A
significant decrease of OTA levels in comparison with the control were observed
with the yeast interaction. The use of live strains with both, beneficial and
bio-control properties, able to prolong the safe storage of feedstuff and exert
beneficial properties after animal consumption, are important to be included in
a novel product to animal feed. This study demonstrated that selected S.
cerevisiae strains exerted in vitro inhibited A. carbonarius. The inhibition
was effective against on both, fungal growth and OTA production, and was
significantly influenced by environmental conditions. The use of S. cerevisiae
RC008 and RC016 as animal feed additives could be an alternative to control
mycotoxigenic fungi in stored feedstuff.