Adpsortion of Zearalenone by the commercial adsorbents Safmannan® Celpec® and Mannsorb®

KELLER K., ; PEREYRA C., ; ALMEIDA T., ; CAVAGLIERI L., ; ROSA C

Tulln, Viena, Austria

Congreso; Global discussion forum on Worldwide Mycotoxin Reduction in Food and Feed Chains; 2009

International Society for Mycotoxicology

Adpsortion of Zearalenone by the commercial adsorbents Safmannan ® Celpec ® and Mannsorb ® Kelly Keller a,b, Carina Pereyra c,d, Tatiana Almeida a,b, Lilia Cavaglieri c,e, and Carlos Rosa a,f a Departamento de Microbiologia e Imunología Veterinária, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro. BR 465 Km 7, Seropédica, Rio de Janeiro, 23890-000, Brazil.  b Fellow of Conselho Nacional de Pesquisas Científicas (CNPq), Brazil. c Departamento de Microbiología e Imnmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto. Ruta 36 km 601 (5800), Río Cuarto, Argentina. d Fellow of Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. e Member of Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. f Member of Conselho Nacional de Pesquisas Científicas (CNPq), Brazil. The purpose of the present study was to evaluate the zearalenone (ZEA) adsorption capacity for three commercial adsorbents: Safmannan ® (YW: yeast wall), Celpec ® (Z: zeolite) and Mannsorb ® (YW + Z) at different pH conditions. Each adsorbent was used at a concentration of 5 mg/ml. A solution of ZEA at 0.25; 0.5; 1; 5; 20 and 50 µg/ml in PBS at pH 2 and pH 6 was prepared. Each adsorbent was incubated for 30 min at 37ºC with 1 ml phosphate-buffered saline (PBS) containing ZEA at different concentrations, separately. They were pelleted by centrifugation and the supernatant containing unbound ZEA was collected. ZEA was quantified by HPLC with fluorescence detector. The results were evaluated according to the model of Bueno et al. (2007) who determined the number of binding sites of mycotoxin (M) and the equilibrium constant involved in the process (Keq). According to this adsorption model Safmannan ® and Celpec ® had high and similar adsorption capacity at the two tested pH. However, Mannsorb ® proved to be more effective at pH 6 since it has an M twice bigger. In addition, the Keq increased ten times, demonstrating a low reversibility of the process and an adsorption of 0.474g of ZEA per gram of adsorbent. Safmannan ® and Celpec ® adsorbed 0.396 and 0.19 g of ZEA per gram of adsorbent, respectively. The laboratory study that we developed can be used to define approximate dose requirements for an adsorbent and define a strategic mechanism for using a particular adsorbent in an animal system to reduce the bioavailability of mycotoxins in animal production systems. Safmannan ®, Celpec ® and Mannsorb ® are attractive supplements for controlling ZEA toxicosis. Studies are being conducted to evaluate their influence on the concentrations of micronutrients and the efficacy on in vivo experiments.