Adsorção ?in vitro? de ochratoxina A por probióticos utilizados na aquicultura

ESCORCIO E.; PEREYRA C.M; ARAÚJO NEVES J; CALVET RM; TEIXEIRA DE OLIVEIRA SANTO J; EVANGELISTA LIMA C; CARVALHO ALVES V; SANCHES MURATORI MC

Acta Veterinaria Brasilica

Universidade Federal Rural do Semi-Árido (UFERSA)

Lugar: Rio Grande do Norte; Año: 2015 vol. 9 p. 59 - 64

Given the constant contamination of animals by feed mycotoxins, this study aimed to evaluate in vitro the ability of commercial products used in aquaculture as adsorbents of ochratoxin A (OTA). Two comercial probiotics, composed of Bacillus subtilis, Bifidobacterium bifidum, Enterococcus faecium and Lactobacillus acidophilus (Probiotic A) and one made of dry yeast of Saccharomyces cerevisiae from a brewery (Probiotic B) were used in the assay. Phosphate buffered saline solutions (PBS) at pH 1.5 and 7.5 were formulated to simulate the pH of the stomach and intestine of Nile Tilapia (Oreochromis niloticus). The PBS solutions were tested at the two pH (1,5 and 7,5) with the probiotics concentrations of 0%; 25%; 50%; 75% and 100% for a OTA concentration of 1.000 ng.mL-1. The OTA concentrations were added in microtubes for evaluation of adsorption capacity of the probiotics. The OTA detection and quantification were performed by High Performance Liquid Chromatography (HPLC). The final results showed that the probiotics A and B in their maximum concentration were capable of adsorbing OTA in amounts from 13.78 to 76.81 and from 301.48 to 317.54 (ng.mL-1), respectively. The data showed that this two probiotics were very promissimg at adsorbing OTA under simulated gastrointestinal conditions of pH of tilapias, being more efficient the probiotic B.