Avaliacao in vitro da adsorção de aflatoxina B1 por produtos comerciais utilizados na alimentação animal

ESCÓRCIO PINHEIRO R.E; PEREYRA C.M; ARAÚJO NEVES J; CALVET R.M; TEIXEIRA DE OLIVEIRA J; EVANGELISTA LIMA C; CARVALHO ALVES V; GOMES PEREIRA M.M; SANCHES MURATORI M.M

Arquivos do Instituto Biologico

Revista ?Arquivos do Instituto Biológico?

Lugar: Sao Paulo; Año: 2017 vol. 84

1808-1657

The aim of this study was to evaluate in vitro aflatoxin B1 (AFB1) adsorption capacity by commercial products used in animal feed. Many studies are being conducted for the decontamination of aflatoxins in feed. There commercial products destined to fish feed that are available as probiotics and are formed by strains of bacteria and yeasts used in most assays adsorption of mycotoxins. Three commercial products were studied: A ? consisting of Bacillus subtilis, Bifidobacterium bifidum, Enterococcus faecium and Lactobacillus acidophilus; B ?consisting of dry yeast of Saccharomyces cerevisiae from brewery and C ? consisting of Bacillus subtilis, Bacillus licheniformis e Bacillus pumilus. Five suspensions of each product (0%, 25%, 50%, 75% and 100%) of dose maximum recommended by the manufacture were tested against AFB1 (1000 ng.mL-1) in microtubes for determine adsorption capacity. To simulate the pH of the stomach and intestine of Nile Tilapia (Oreochromis niloticus), phosphate buffered saline solutions (PBS) at pH 1.5 and 7.5, respectively, were formulated. Microtubes were introduced into a centrifuge with mechanical agitation at 37°C for one hour and then centrifuged for 10 min at 14000 rpm; the supernatants were quantified by high-performance liquid chromatography (HPLC). The commercial products in the maximum concentration were capable of adsorbing AFB1 in amounts from 45.01 to 129.59; from 123.9 to 215.59 and from 209.98 to 370.73 (ng.mL-1), respectively. It was concluded that all commercial products, which are added to animal feed, adsorbed AFB1 under simulated gastrointestinal pH conditions and are potential candidates for AFB1 adsorption for future studies in vivos.