ASSESSMENT OF LACTIC BACTERIA INTERACTION WITH FOOD ANTINUTRITIONAL COMPONENTS TO PREVENT THEIR DELETERIOUS EFFECTS ON THE HOST

SAEZ, G.D.; PALACIOS J.M. ; ZÁRATE G.

Congreso; XXX ANNUAL SCIENTIFIC MEETING OF TUCUMAN BIOLOGY ASSOCIATION; 2014

Asociación de Biología de Tucumán

Many antinutritional or toxic compounds are ingested daily by humans and animals. Vegetable lectins are endogenous proteins present in legumes, cereals and seeds whereas aromatic amines are generated by processing of meat. Since these substances resist inactivation by cooking and digestive processes, they reach the intestinal lumen and blood circulation unaltered. Their toxic effects are evidenced by morphological and physiological changes in the intestinal mucosa and genotoxic effects on colonocytes. It has been demonstrated that probiotics can remove and metabolize carcinogens and antinutritional compounds from food and from the gut, decreasing their deleterious effects on the host. In this work we assessed the interaction of potentially probiotic bacteria with 2-amino-3-methyl-3H-imidazo[4,5-f]quinoline (IQ) or the lectin of Phaseolus vulgaris in order to remove and prevent their toxicity on intestinal cells. Four propionibacteria and lactobacilli were incubated with these substances before assessing the cyto- and genotoxicity of the reaction supernatants on mice colonocytes. IQ was significantly more toxic than kidney lectin and no strain was able to remove it. In contrast, all strains were ablo to bind and remove lectin, decreasing its toxic effects on enterocytes.