Impairment of Listeria monocytogenes biofilm developed on industrial surfaces by Latilactobacillus curvatus CRL1579 bacteriocin

MELIAN, CONSTANZA; PLOPER, DIEGO; CHEIN, ROXANA; VIGNOLO, GRACIELA; CASTELLANO, PATRICIA

FOOD MICROBIOLOGY

ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2024 vol. 121

0740-0020

The effect of lactocin AL705, bacteriocin produced by Latilactobacillus (Lat.) curvatus CRL1579 against Listeriabiofilms on stainless steel (SS) and polytetrafluoroethylene (PTFE) coupons at 10 ◦C was investigated.L. monocytogenes FBUNT showed the greatest adhesion on both surfaces associated to the hydrophobicity of cellsurface. Partially purified bacteriocin (800 UA/mL) effectively inhibited L. monocytogenes preformed biofilmthrough displacement strategy, reducing the pathogen by 5.54 ± 0.26 and 4.74 ± 0.05 log cycles at 3 and 6 days,respectively. The bacteriocin-producer decreased the pathogen biofilm by ~2.84 log cycles. Control and Bac−treated samples reached cell counts of 7.05 ± 0.18 and 6.79 ± 0.06 log CFU/cm2 after 6 days of incubation.Confocal scanning laser microscopy (CLSM) allowed visualizing the inhibitory effect of lactocin AL705 onL. monocytogenes preformed biofilms under static and hydrodynamic flow conditions. A greater effect of thebacteriocin was found at 3 days independently of the surface matrix and pathogen growth conditions at 10 ◦C. Asa more realistic approach, biofilm displacement strategy under continuous flow conditions showed a significantloss of biomass, mean thickness and substratum coverage of pathogen biofilm. These findings highlight the antibiofilm capacity of lactocin AL705 and their potential application in food industries.