Administration of probiotic lactic acid bacteria to modulate fecal microbiome in feedlot cattle

MANSILLA, F.I.; ARISTIMUÑO FICOSECO, M.C.; MIRANDA, M.H.; PUGLISI, E.; NADER-MACIAS, M.E.F; VIGNOLO, G; FONTANA, C.

Scientific Reports

Nature Publishing Group

Lugar: Londres; Año: 2022 vol. 12 p. 1 - 16

2045-2322

Modulation of animal gut microbiota is a prominent function of probiotics to improve the healthand performance of livestock. In this study, a large-scale survey to evaluate the efect of lactic acidbacteria probiotics on shaping the fecal bacterial community structure of feedlot cattle during threeexperimental periods of the fattening cycle (163 days) was performed. A commercial feedlot located innorthwestern Argentina was enrolled with cattle fed mixed rations (forage and increasing grain diet)and a convenience-experimental design was conducted. A pen (n= 21 animals) was assigned to eachexperimental group that received probiotics during three diferent periods. Groups of n= 7 animalswere sampled at 40, 104 and 163 days and these samples were then pooled to one, thus giving a totalof 34 samples that were subjected to high-throughput sequencing. The microbial diversity of fecalsamples was signifcantly afected (p < 0.05) by the administration period compared with probioticgroup supplementation. Even though, the three experimental periods of probiotic administrationinduced changes in the relative abundance of the most representative bacterial communities,the fecal microbiome of samples was dominated by the Firmicutes (72–98%) and Actinobacteria(0.8–27%) phyla, while a lower abundance of Bacteroidetes (0.08–4.2%) was present. Probioticswere able to modulate the fecal microbiota with a convergence of Clostridiaceae, Lachnospiraceae,Ruminococcaceae and Bifdobacteriaceae associated with health and growth benefts as coremicrobiome members. Metabolic functional prediction comparing three experimental administrationperiods (40, 104 and 163 days) showed an enrichment of metabolic pathways related to complexplant-derived polysaccharide digestion as well as amino acids and derivatives during the frst 40 daysof probiotic supplementation. Genomic-based knowledge on the benefts of autochthonous probioticson cattle gastrointestinal tract (GIT) microbiota composition and functions will contribute to theirselection as antibiotic alternatives for commercial feedlot.