INVESTIGADORES
GRAMAJO Hugo Cesar
congresos y reuniones científicas
Título:
Transcriptional activation of the fatty acid biosynthetic genes in Streptomyces coelicolor
Autor/es:
COMBA S., D'ANGELO, M., ARABOLAZA, A. GRAMAJO. H
Lugar:
Huerta Grande-Cordoba
Reunión:
Congreso; XLIV Reunión Anual de la Sociedad Argentina de Bioquímica y Biología Molecular (SAIB; 2008
Institución organizadora:
SAIB
Resumen:
Fatty acid biosynthesis is not only an essential process for most
living cells, but it is also energetically expensive. Thus organisms
have developed homeostatic mechanisms that maintain the
concentration of lipids at particular levels. In Streptomyces
coelicolor the essential FAS genes are clustered at a single location
within its genome (fab operon). Bioinformatics analysis of the fab
genes locus revealed the presence of a highly conserved open
reading frame located upstream of fabD, named fasA, which has
homology with transcriptional regulators. To investigate the
functional role of FasA in the transcriptional regulation of the fab
operon, we initially constructed a fasA disruption mutant (AM6).
This strain showed an evident reduction in vegetative growth rate
and a delay in the onset of sporulation. AM6 also presented
diminished FAS activity, distorted composition of total fatty acids
and reduced triacylgliceride content compared to the wild type
strain. Further the analysis of a transcriptional fusion to fabD
showed an 8-fold reduction in its expression in AM6 cells. To
confirm that this effect was exclusively related to the absence of
FasA, we complemented the AM6 mutant. In this genetic
background the fabD transcription, as well as the growth rate, was
restored to the wild type levels. All these results suggest that FasA
is involved in transcriptional activation of fab operon.
santicomba@gmail.com