INVESTIGADORES
GRAMAJO Hugo Cesar
artículos
Título:
Recombinant Protein Production in High Cell Density Cultures of Escherichia coli with Galactose as a Gratuitous Inducer
Autor/es:
HUGO G. MENZELLA AND HUGO C. GRAMAJO
Revista:
BIOTECHNOLOGY PROGRESS
Referencias:
Año: 2004 p. 1263 - 1266
ISSN:
8756-7938
Resumen:
A new expression system was developed by introducing two major
modifications into the genome of Escherichia coli: a deletion in the
gal operon (DeltagalEKT) to allow the use of the inexpensive compound
galactose as a gratuitous inducer and the introduction of the gal P2
promoter driving the expression of the T7 RNA polymerase. The novel
JRR10 strain containing these two features gives high-level expression
of a reporter gene cloned under the T7 phi10 promoter in high cell
density cultures. The cost of the induction of this novel system is
more than 30 times lower than that of the IPTG-induced system of the
widely used BL21 strain.