INVESTIGADORES
GRAMAJO Hugo Cesar
artículos
Título:
Transcriptional regulation of fatty acid biosynthesis in mycobacteria.
Autor/es:
. MONDINO S, GAGO G, GRAMAJO H
Revista:
MOLECULAR MICROBIOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2013 vol. 89 p. 372 - 387
ISSN:
0950-382X
Resumen:
The main purpose of our study is to understand how
mycobacteria exert control over the biosynthesis of
their membrane lipids and find out the key components
of the regulatory network that control fatty acid
biosynthesis at the transcriptional level. In this article
we describe the identification and purification of FasR,
a transcriptional regulator from Mycobacterium sp.
that controls the expression of the fatty acid synthase
(fas) and the 4-phosphopantetheinyl transferase
(acpS) encoding genes, whose products are involved
in the fatty acid and mycolic acid biosynthesis pathways.
In vitro studies demonstrated that fas and acpS
genes are part of the same transcriptional unit and that
FasR specifically binds to three conserved operator
sequences present in the fas-acpS promoter region
(Pfas). The construction and further characterization
of a fasR conditional mutant confirmed that FasR is a
transcriptional activator of the fas-acpS operon and
that this protein is essential for mycobacteria viability.
Furthermore, the combined used of Pfas?lacZ fusions
in different fasR backgrounds and electrophoretic
mobility shift assays experiments, strongly suggested
that long-chain acyl-CoAs are the effector molecules
that modulate the affinity of FasR for its DNA binding
sequences and therefore the expression of the essential
fas-acpS operon.