Development of Double Emulsion Nanoparticles for the Encapsulation of Bovine Serum Albumin

MARTINEZ, NELIDA.Y.; ANDRADE, PATRICIA F.; DURAN, NELSON; CAVALITTO, SEBASTIAN

COLLOIDS AND SURFACES B-BIOINTERFACES

ELSEVIER SCIENCE BV

Año: 2017 vol. 158 p. 190 - 196

0927-7765

In the present work, a double emulsion was developed for the encapsulation of BovineSerum Albumin (BSA) as a model protein for the future encapsulation of viral proteins.The first emulsion polydispersity index (PDI) was studied with increasing concentrationsof poly (ε-caprolactone) (PCL) as stabilizer (from 16 % w/v to 5 % w/v) and polyvinylalcohol (PVA) as the surfactant in the second emulsion at 1.5 % w/v. Results suggest thatat decreasing concentrations of PCL the PDI of the emulsion also decrease, indicatingthat viscosity of the emulsion is crucial in the homogeneity of the resultant sizedistribution of the nanoparticles. When PVA concentration in the second emulsion wasincreased from 1.5 % w/v to 2.5 % w/v the PDI also increased. To study the relationshipbetween the structure of the surfactant in the second emulsion and the resultant BSAencapsulation, emulsions were prepared with Pluronic F68 and PVA both at 1.5 % w/vand PCL in the first emulsion at 5 % w/v. Results indicated that Pluronic F68 was a betterstabilizer because at the same experimental conditions encapsulation of BSA was 1.5higher than PVA. FTIR studies confirmed the presence of BSA in the nanoparticles. SEMand TEM microscopies showed a size distribution of 300 nm to 500 nm size ofnanoparticles. Circular dichroism studies demonstrated that the secondary structure of theprotein was conserved after the encapsulation into the nanoparticles.