Evaluation of agro-industrial wastes, their state and mixing ratio for maximum polygalacturonase and biomass production in submerged fermentation

GOGUS, NIHAN; EVCAN, EZGI; TARI, CANAN; CAVALITTO, SEBASTIÁN FERNANDO

ENVIRONMENTAL TECHNOLOGY

TAYLOR & FRANCIS LTD

Lugar: Londres; Año: 2015 vol. 36 p. 2657 - 2667

0959-3330

The potential of important agro-industrial wastes; apple pomace (AP) and orange peel (OP) as C sources, was investigated in the maximization of polygalacturonase (PG), an industrially significant enzyme, using an industrially important microorganism Aspergillus sojae. Factors such as various hydrolysis forms of the C sources (hydrolyzed-AP, nonhydrolyzed-AP, hydrolyzed-AP+OP, nonhydrolyzed- AP+OP), N sources (ammonium sulphate and urea) and incubation time (4, 6, 8 days) were screened. It was observed that maximum PG activity was achieved at a combination of non-hydrolyzed-AP+OP and ammonium sulphate with 8 days of incubation. For the pre-optimization study, ammonium sulphate concentration and the mixing ratios of AP+OP at different total C concentrations (9, 15, 21 g l -1 ) were evaluated. The optimum conditions for the maximum PG production (144.96 U ml -1 ) was found as 21 g l -1 total carbohydrate concentration totally coming from OP at 15 g l -1 ammonium sulphate concentration. On the other hand 3:1 mixing ratio of OP+AP at 11.50 g/l ammonium sulphate concentration also resulted into a considerable PG activity (115.73 U ml -1 ). These results demonstrated that AP can be evaluated as an additional C source to OP for PG production, which in turn both can be alternative solutions for the elimination of the waste accumulation in the food industry with economical returns.