INVESTIGADORES
ALARCON Sergio Hugo
congresos y reuniones científicas
Título:
Isolation of bacterial strains with lipolitic activity from dairy industry waste waters.
Autor/es:
IGNACIO SANTECCHIA; FLORENCIA FICARRA; SERGIO ALARCON; CHRISTIAN MAGNI; MARTÍN ESPARIZ
Reunión:
Congreso; L Reunión Anual. Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2014
Resumen:
Dairy industries generate up to 10 L of wastewater per
liter of processed milk causing a significant environmental problem. Since these wastewaters are rich in biodegradable
organic molecules, their treatment usually involves bacteria addition. The aim
of this work was to isolate bacterial strains for their use in the degradation
of dairy wastewater fats. With this attempt, lipase producing strains were screened
from a wastewater treatment lagoon of a local dairy industry. Exoenzyme
profiles were performed for 58 isolates. Then, phylogenetic clustering of 49
strains was performed by RAPD analysis. 10 strains were evaluated for their
capability to adapt to wastewater specific environmental conditions. For this, selected isolates were grown individually in sterile
wastewater medium supplemented with glucose or oil as carbon source. Lipolytic
activities in supernatants and bacterial growth rate (µ) were monitored. Four
strains showed a µmax higher than the mean µmax in both
media. Finally, 16S rRNA and/or housekeeping gene sequences of selected strains
were determined. We identified strains with highest shared identity with Bacillus, Desemzia,
Aeromonas, and Acinetobacter species. In summary, in this work we select strains based on
their true-lipase activity against low degradability rate triglycerides and
their suitable growth in wastewater media.