Genome mining of lipolytic exoenzymes from Bacillus safensis S9 and Pseudomonas alcaliphila ED1 isolated from a dairy wastewater lagoon

FLORENCIA FICARRA; IGNACIO SANTECCHIA; SEBASTIÁN LAGORIO; SERGIO ALARCON; CHRISTIAN MAGNI; MARTÍN ESPARIZ

ARCHIVES OF MICROBIOLOGY

SPRINGER

Lugar: Berlin; Año: 2016

0302-8933

Dairy production plants produce highly pollutedwastewaters rich in organic molecules such as lactose, proteinsand fats. Fats generally lead to low overall performanceof the treatment system. In this study, a wastewaterdairy lagoon was used as microbial source and differentscreening strategies were conducted to select 58 lipolyticmicroorganisms. Exoenzymes and RAPD analyses revealedgenetic and phenotypic diversity among isolates. Bacillussafensis, Pseudomonas alcaliphila and the potentialpathogens, B. cereus, Aeromonas and Acinetobacter wereidentified by 16S-rRNA, gyrA, oprI and/or oprL sequenceanalyses. Five out of 10 selected isolates produced lipolyticenzymes and grew in dairy wastewater. Based onthese abilities and their safety, B. safensis S9 and P. alcaliphilaED1 were selected and their genome sequencesdetermined. The genome of strain S9 and ED1 consistedof 3,794,315 and 5,239,535 bp and encoded for 3990 and4844 genes, respectively. Putative extracellular enzymeswith lipolytic (12 and 16), proteolytic (20) or hydrolytic(10 and 15) activity were identified for S9 and ED1 strains,respectively. These bacteria also encoded other technologicalrelevant proteins such as amylases, proteases, glucanases,xylanases and pectate lyases.