Development of an Electrochemical Immunosensor for Ochratoxin A

ALARCON SERGIO; MICHELI LAURA; PALLESCHI GIUSEPPE; COMPAGNONE DARIO

Analytical Letters

Taylor & Francis

Lugar: Filadelfia; Año: 2004 vol. 37 p. 1545 - 1558

A direct, competitive electrochemical enzyme-linked immunosorbent assay (ELISA) has been developed for the quantitative determination of ochratoxin A (OTA) using polyclonal antibodies. The assay is carried out on carbon-based screen printed electrodes (SPE). Optimisation of the ELISA competitive conditions allowed us to realise an assay with improved analytical behaviour compared to the classical spectrophotometric ELISA based assay. The performance was comparable to a published monoclonal based assay. The assay gave a detection limit of 180 pgmL21 and sensitivity of 6.1+0.1 ngmL21. The immunosensor was challenged with wine to assess a matrix effect. Recoveries obtained were in the 70?118% range. The method appears to be suitable for OTA contamination screening in food samples. was challenged with wine to assess a matrix effect. Recoveries obtained were in the 70?118% range. The method appears to be suitable for OTA contamination screening in food samples. was challenged with wine to assess a matrix effect. Recoveries obtained were in the 70?118% range. The method appears to be suitable for OTA contamination screening in food samples. published monoclonal based assay. The assay gave a detection limit of 180 pgmL21 and sensitivity of 6.1+0.1 ngmL21. The immunosensor was challenged with wine to assess a matrix effect. Recoveries obtained were in the 70?118% range. The method appears to be suitable for OTA contamination screening in food samples. was challenged with wine to assess a matrix effect. Recoveries obtained were in the 70?118% range. The method appears to be suitable for OTA contamination screening in food samples. was challenged with wine to assess a matrix effect. Recoveries obtained were in the 70?118% range. The method appears to be suitable for OTA contamination screening in food samples. published monoclonal based assay. The assay gave a detection limit of 180 pgmL21 and sensitivity of 6.1+0.1 ngmL21. The immunosensor was challenged with wine to assess a matrix effect. Recoveries obtained were in the 70?118% range. The method appears to be suitable for OTA contamination screening in food samples. was challenged with wine to assess a matrix effect. Recoveries obtained were in the 70?118% range. The method appears to be suitable for OTA contamination screening in food samples. was challenged with wine to assess a matrix effect. Recoveries obtained were in the 70?118% range. The method appears to be suitable for OTA contamination screening in food samples. published monoclonal based assay. The assay gave a detection limit of 180 pgmL21 and sensitivity of 6.1+0.1 ngmL21. The immunosensor was challenged with wine to assess a matrix effect. Recoveries obtained were in the 70?118% range. The method appears to be suitable for OTA contamination screening in food samples. was challenged with wine to assess a matrix effect. Recoveries obtained were in the 70?118% range. The method appears to be suitable for OTA contamination screening in food samples. was challenged with wine to assess a matrix effect. Recoveries obtained were in the 70?118% range. The method appears to be suitable for OTA contamination screening in food samples. was challenged with wine to assess a matrix effect. Recoveries obtained were in the 70?118% range. The method appears to be suitable for OTA contamination screening in food samples.