The production of yeast cell wall using an agroindustrial waste influences the wall thickness and is implicated on the aflatoxin B 1 adsorption process

PEREYRA, C.M.; GIL, S.; CRISTOFOLINI, A.; BONCI, M.; MAKITA, M.; MONGE, M.P.; MONTENEGRO, M.A.; CAVAGLIERI, L.R.

FOOD RESEARCH INTERNATIONAL

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2018

0963-9969

The objectives of this study were: to evaluate the use of dry distillery grain soluble extract -DDGse to produce yeast biomass and to obtain cell wall (CW), to use the CW as anaflatoxin B1 (AFB1) adsorbent, to study the variation in the composition and thickness ofthe CW under the influence of DDGse to evaluate their implication on the adsorptionprocess using transmission electron microscopy (TEM) and fourier-transform infraredspectroscopy (FITR). The production of biomass and CW were variable. The CW thicknessvalues showed that S. boulardii strain grown in yeast extract peptone dextrose (YPD) orDDGse medium, with no significant differences observed. The thickness of the CW for S.cerevisiae (RC012 and VM014) were increased when the cells were grown in DDGsemedium, the thickness was almost double compared to the values obtained in YPDmedium. The spectra IR of each CW in the two culture media shown regions correspondingto polysaccharides, proteins and lipids. Cells grown in DDGse medium adsorbed moreAFB1 than those grown in YPD. The CW adsorbed more AFB1 than the same amount ofwhole cell. Future studies should be done to determine the type of carbohydrates and therelationship between chitin - beta glucans responsible for mycotoxin adsorption.