INVESTIGADORES
CHIRDO Fernando Gabriel
congresos y reuniones científicas
Título:
Analysis of draining leukocytes from the abdominal cavity monitors Immune events after intestinal transplant.
Autor/es:
MEIER D; CAGNOLA H; RAMISCH C; RUMBO C; DOCENA G; CHIRDO F; GONDOLESI G; RUMBO M
Lugar:
Buenos Aires
Reunión:
Congreso; 1st French Argentine Immunology Congress SAI-FAIC; 2010
Institución organizadora:
Sociedad Argentina de Inmunologia
Resumen:
During intestinal transplant (ITx)
operation, intestinal lymphatics are not reconstituted. Consequently, migrating
immune cells drain freely into the abdominal cavity and could be recovered
though a drainage of the intestinal cavity used for clinical postoperative
surveillance. Our aim was to evaluate whether leukocytes migrating from the
transplanted intestine could be recovered from the abdominal draining fluid and
to determine potential applications of the assessment of draining cellular
populations to basic and clinical studies. Draining fluid was collected by a
peritoneal drainage system in the early postoperative period of 7 ITx patients.
Cell composition of the abdominal draining fluid was analyzed by flow cytometry
during the first 14 post-ITx days. Cell sorting and molecular fingerprinting by
short tandem repeat amplification was performed. The correlation between
analyzed parameters and clinical evolution was evaluated. The main populations
in the draining fluid were CD3+CD4+CD8-, CD3+CD8+CD4- and CD3-HLA-DR+CD19+
lymphocytes, although several minor populations were identified. Cellular
pattern varies along the post-ITx period in non-complicated recipients from a
mixed leukocyte pattern to an exclusively lymphocytic pattern. We could
associate changes in draining cell patterns to early rejection or infections.
Graft derived lymphocytes were recognized by genetic fingerprinting of CD8+
sorted T cells. At days 1-2, donor T cells were detected in the draining fluid
(50% of total CD8+ cells) and were mostly replaced by day 11 after ITx
(<2%), confirming that cells migrating from the graft can be recovered in
the draining fluid.This study demonstrates that cell
analysis of the draining fluid from ITx recipients may provide a useful
approach for monitoring changes in graft immunobiology during the first 3 weeks
post-transplant. Furthermore, this is a unique opportunity to study different
immune cell populations migrating from the mucosal intestinal site.