INVESTIGADORES
CHIRDO Fernando Gabriel
congresos y reuniones científicas
Título:
Considerations concerning methods for gluten quantitation in foods (R5/G12 ELISA).
Autor/es:
KOHLER P; CHIRDO FG; DEUSTCH H; MOTHES T; TRANQUET O; SCHERF K
Lugar:
Valencia
Reunión:
Simposio; 30th Meeting of the European Prolamin Working Group of Prolamin Analysis and Toxicity.; 2016
Institución organizadora:
ProcEuropean Prolamin Working Group of Prolamin Analysis and Toxicity.
Resumen:
The sandwich ELISA based on the R5 monoclonal antibody [1] for the quantitation of intact gluten was endorsed as a Codex Alimentarius type 1 method [2] and has also been adopted as AOAC International [3] and AACC International [4] approved methods. Rice- and maize-based matrices have been used in these studies. A competitive version of the R5 ELISA has also been approved by both standardisation organisations [5,6]. In 2008, sandwich and competitive ELISAs based on the G12 (and A1) monoclonal antibodies were developed [7] and are now commercially available. The G12 sandwich ELISA has also been validated in an international collaborative study using rice-based matrices and is approved as AACC International [8] and AOAC International [9] method.
