INVESTIGADORES
CHIRDO Fernando Gabriel
artículos
Título:
Analysis of immune cells draining from the abdominal cavity as a novel tool to study intestinal transplant immunobiology
Autor/es:
MEIER D; CAGNOLA H; RAMSICH D; RUMBO C; CHIRDO F; DOCENA G; GONDOLESI G; RUMBO M
Revista:
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2010 vol. 162 p. 138 - 145
ISSN:
0009-9104
Resumen:
During intestinal transplant (ITx) operation, intestinal lymphatics are
not reconstituted. Consequently, trafficking immune cells drain freely into the
abdominal cavity. Our aim was to evaluate whether leucocytes migrating from a
transplanted intestine could be recovered from the abdominal draining fluid
collected by a peritoneal drainage system in the early post-ITx period, and to
determine potential applications of the assessment of draining
cellular populations. The cell composition of the abdominal draining fluid
was analysed during the first 11 post-ITx days. Using flow cytometry, immune
cells from blood and draining fluid samples obtained the same day showed an
almost complete lymphopenia in peripheral blood, whereas CD3+CD4+CD8-, CD3+CD4-CD8+ and human leucocyte
antigen D-related (HLA-DR)+CD19+ lymphocytes were the main
populations in the draining fluid. Non-complicated recipients evolved from a mixed leucocyte pattern
including granulocytes, monocytes and lymphocytes to an exclusively lymphocytic
pattern along the first post-ITx week. At days 12 post-Itx, analysis by short
tandem repeats fingerprinting of CD3+CD8+ sorted T cells from draining fluid indicated that 50% of
cells were from graft origin, whereas by day 11 post-ITx this proportion
decreased to fewer than 1%. Our results show for the first time that the abdominal drainage fluid contains
mainly immune cells trafficking from the implanted intestine, providing the
opportunity to sample lymphocytes draining from the grafted organ along the post-ITx
period. Therefore, this analysis may provide information useful for understanding
ITx immunobiology and eventually could also be of interest for clinical
management.