Mucosal Tissue Transglutaminase expression in celiac disease

VILLANACI V; NOT T; SBLATTERO D; GAIOTTO T; CHIRDO F; GALLETI A; BASSOTTI G

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (PRINT)

Wiley

Lugar: London; Año: 2009 p. 334 - 340

1582-1838

<!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:IT; mso-fareast-language:IT;} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> <!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-GB;} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Aims. To investigate the localization of tTG in the duodenal mucosa in control tissues and in different histological stages of celiac disease by using commercial and a novel set of anti-tTG monoclonal antibodies, to see whether this assessment can be useful for diagnostic purpose. Patients and Methods. The distribution of tTG was firstly evaluated in 18 celiac patients by using a commercial monoclonal antibody (CUB7402) against guinea-pig type tissue transglutaminase enzyme and directed against the loop-core region of the enzyme. Thereafter, in further 30 celiac patients we employed three newly characterized anti-tTG monoclonal antibodies produced against recombinant human-tTG. The epitopes recognized are located in three distinct domains of the protein corresponding to the core, C1 and C2 protein structure. Eleven age- and sex-matched patients with chronic duodenitis acted as controls. All subjects underwent upper endoscopy to obtain biopsy samples from the duodenum. Results. Overall, we found that a) tTG is equally expressed in CD at different stages of disease; b) tTG is expressed, at similar level, in CD and controls with duodenitis. Conclusions. Assessment of tTG level in biopsy samples by immunohistochemical methods is not useful in the clinical diagnostic work-up of CD.