MURINE IMMUNE RESPONSE TO INTRANASAL IMMUNIZATION WITH A COMPONENT OF THE TYPE IV SECRETION SYSTEM FROM Brucella abortus

ANDREA FERNANDEZ; MARIANA CRISTINA FERRERO; MARIA SOLEDAD HIELPOS; IVAN MATHIAS ALONSO PAIVA; FLORENCIA MUÑOZ GONZÁLEZ; JULIANA FALIVENE; PABLO C. BALDI

Nairobi

Encuentro; International Veterinary Vaccinology Network Meeting; 2018

Universidad de Edimburgo

Brucellosis is usually transmitted to humans through mucosae. Currently, there are no humanvaccine against this disease. We decided to evaluate a virulence factor of Brucella (VirB7) as apotential component of an acellular vaccine against brucellosis, in a murine model.Six week-old Balb/c mice were weekly immunized for 3 weeks with VirB7 (10ug) plus choleratoxin (CT, 2ug), CT alone or saline. One week after last immunization serum, saliva, feces,bronchoalveolar (BAL) and vaginal lavage fluids (LV), lung homogenates and spleens wereobtained. Levels of VirB7-specific antibodies were measured by indirect ELISA in all samples. Invitro production of gamma interferon (IFN-γ), interleukin 2 (IL-2) and IL-5 by spleen cellsstimulated with VirB7 (1 or 10 µg), ConA or RPMI was determined. Other mice (VirB7 plus CT)were injected intradermally in opposite footpads with VirB7 or saline to evaluate DTH.Immunized mice showed increased serum levels of total antibodies against VirB7 (p