EFFECTS OF SAGS OF THE EGC OPERON ON IMMUNE INNATE CELLS

MALCHIODI, EMILIO LUIS; SARRATEA, MARÍA BELÉN; FERNÁNDEZ MARISA M; IANNANTUONO LOPEZ, LAURA; TOWSTYKA, NADIA YASMÍN; JANCIC, CAROLINA; ANTONOGLOU, MARÍA BELÉN; NOLI TRUANT, SOFÍA

CABA

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

SAI-SAIC-SAFIS-SAB-SAP-SAFE

Bacterial superantigens (SAgs) are enterotoxins that bind to MHC-II and TCR, activating as much as 20% of T cells and promoting a cytokine storm which enhances endotoxic shock and produces immunosuppression, hindering the immune response against bacterial infection. The egc operon reported in autochthonous S. aureus strains includes natural variants of the SAgs genes seg, sei, sem, sen and seo. We have previously characterized the complementary activity of SEG, SEI, SEO and SEM, on phagocytic cells, and reported a M1 response tendency. In the present work we investigated if other cells of the innate immunity are responsible for this pro inflamatory profile. Thus, we evaluated the SAgs effect on human purified γδ T cells from healthy donors by flow citometry and ELISA. We found that, in contrast to αβ T cells response, SAgs activate γδ T cells from 0.01 μM (p