ROLE OF HYALURONAN (HA) AND ITS RECEPTOR RHAMM IN PROLIFERATION, MIGRATION AND DIFFERENTIATION USING JEG-3 CHORIOCARCINOMA CELL LINE. CHARACTERIZATION OF ENZYMES RELATED WHIT HA METABOLISMS

DIAZ M.; PAPADEMETRIO D,; HAJOS S; LOMPARDÍA SILVINA; LEVERMAN MARTIN; MASCARO M,; PIBUEL M; MIHALEZ C; ALVAREZ ELIDA

Mar del Plata

Congreso; Reunion Anual SAIC; 2016

SAIC- SAI- SAFE

Choriocarcinoma (CC) is an aggressive neoplasia that affects women in fertile age. Although molecular aspects of this pathology have been studied those related with extracellular matrix have not been totally clarified. The aim of this work was to evaluate the expression and involvement of HA and RHAMM in CC biology. Human JAR, JEG-3, BeWo and rat Rcho CC cell lines were used. HA secretion was measured by ELISA-like assay. RHAMM expression was determined by western blot and IFI. RHAMM, HA synthases (HAS) and hyaluronidases (Hyals) mRNA expression were evaluated by RT-PCR. The effect of several molecular weight HA (high, low, and oligomers) in migration, proliferation and differentiation (HLA-G and Syncitin-2 mRNA expression) was determined by wound healing and transwell assays, [3H]-thymidine incorporation and RT-PCR, respectively. Ly294002 (PI3K inhibitor) and UO126 (MEK1/2 inhibitor) were used to evaluate signalling pathways involved using JEG-3 cell line. All cell lines studied showed HAS1-2 and Hyals1-2 expression while only BeWo showed higher basal secretion of HA respect to its cell-free medium (p