IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
artículos
Título:
In vitro production of llama (Lama glama) embryos by IVF and ICSI with fresh semen
Autor/es:
PAMELA A. CONDE, CAROLINA HERRERA, VIRGINIA L. TRASORRAS, SUSANA M. GIULIANO, ARIEL DIRECTOR, MARCELO H. MIRAGAYA, MARÍA G. CHAVES, MARÍA I. SARCHI, DIANA STIVALE, CARLOS QUINTANS, ALICIA AGUERO, BRUNO RUTTER, SERGIO PASQUALINI
Revista:
ANIMAL REPRODUCTION SCIENCE
Editorial:
ELSEVIER
Referencias:
Año: 2007
ISSN:
0378-4320
Resumen:
The interest for South American camelids has increased in the last years. The aim of the present research
was to compare the in vitro production of Lama glama embryos using two techniques: in vitro fertilization
(IVF) and intracytoplasmic sperm injection (ICSI). For IVF technique, we compared the effect of adding or
not, heparin, penicillamine and hypotaurine as sperm capacitating agents. In the oocyte group subjected to
ICSI, activation with or without, ionomycin and 6-dimethylaminopurine (6-DMAP) was assessed. Semen
samples were obtained by electroejaculation and incubated at 38 ◦C in a 25% (v/v) collagenase solution. The
cleavage and embryo development rates were compared between the different experimental groups. Only
the number of cleaved oocytes was less when ICSI with no activation was used (p < 0.05).
cleavage and embryo development rates were compared between the different experimental groups. Only
the number of cleaved oocytes was less when ICSI with no activation was used (p < 0.05).
cleavage and embryo development rates were compared between the different experimental groups. Only
the number of cleaved oocytes was less when ICSI with no activation was used (p < 0.05).
cleavage and embryo development rates were compared between the different experimental groups. Only
the number of cleaved oocytes was less when ICSI with no activation was used (p < 0.05).
(IVF) and intracytoplasmic sperm injection (ICSI). For IVF technique, we compared the effect of adding or
not, heparin, penicillamine and hypotaurine as sperm capacitating agents. In the oocyte group subjected to
ICSI, activation with or without, ionomycin and 6-dimethylaminopurine (6-DMAP) was assessed. Semen
samples were obtained by electroejaculation and incubated at 38 ◦C in a 25% (v/v) collagenase solution. The
cleavage and embryo development rates were compared between the different experimental groups. Only
the number of cleaved oocytes was less when ICSI with no activation was used (p < 0.05).
cleavage and embryo development rates were compared between the different experimental groups. Only
the number of cleaved oocytes was less when ICSI with no activation was used (p < 0.05).
cleavage and embryo development rates were compared between the different experimental groups. Only
the number of cleaved oocytes was less when ICSI with no activation was used (p < 0.05).
cleavage and embryo development rates were compared between the different experimental groups. Only
the number of cleaved oocytes was less when ICSI with no activation was used (p < 0.05).
(IVF) and intracytoplasmic sperm injection (ICSI). For IVF technique, we compared the effect of adding or
not, heparin, penicillamine and hypotaurine as sperm capacitating agents. In the oocyte group subjected to
ICSI, activation with or without, ionomycin and 6-dimethylaminopurine (6-DMAP) was assessed. Semen
samples were obtained by electroejaculation and incubated at 38 ◦C in a 25% (v/v) collagenase solution. The
cleavage and embryo development rates were compared between the different experimental groups. Only
the number of cleaved oocytes was less when ICSI with no activation was used (p < 0.05).
cleavage and embryo development rates were compared between the different experimental groups. Only
the number of cleaved oocytes was less when ICSI with no activation was used (p < 0.05).
cleavage and embryo development rates were compared between the different experimental groups. Only
the number of cleaved oocytes was less when ICSI with no activation was used (p < 0.05).
cleavage and embryo development rates were compared between the different experimental groups. Only
the number of cleaved oocytes was less when ICSI with no activation was used (p < 0.05).
(IVF) and intracytoplasmic sperm injection (ICSI). For IVF technique, we compared the effect of adding or
not, heparin, penicillamine and hypotaurine as sperm capacitating agents. In the oocyte group subjected to
ICSI, activation with or without, ionomycin and 6-dimethylaminopurine (6-DMAP) was assessed. Semen
samples were obtained by electroejaculation and incubated at 38 ◦C in a 25% (v/v) collagenase solution. The
cleavage and embryo development rates were compared between the different experimental groups. Only
the number of cleaved oocytes was less when ICSI with no activation was used (p < 0.05).
cleavage and embryo development rates were compared between the different experimental groups. Only
the number of cleaved oocytes was less when ICSI with no activation was used (p < 0.05).
cleavage and embryo development rates were compared between the different experimental groups. Only
the number of cleaved oocytes was less when ICSI with no activation was used (p < 0.05).
cleavage and embryo development rates were compared between the different experimental groups. Only
the number of cleaved oocytes was less when ICSI with no activation was used (p < 0.05).
in vitro production of Lama glama embryos using two techniques: in vitro fertilization
(IVF) and intracytoplasmic sperm injection (ICSI). For IVF technique, we compared the effect of adding or
not, heparin, penicillamine and hypotaurine as sperm capacitating agents. In the oocyte group subjected to
ICSI, activation with or without, ionomycin and 6-dimethylaminopurine (6-DMAP) was assessed. Semen
samples were obtained by electroejaculation and incubated at 38 ◦C in a 25% (v/v) collagenase solution. The
cleavage and embryo development rates were compared between the different experimental groups. Only
the number of cleaved oocytes was less when ICSI with no activation was used (p < 0.05).
cleavage and embryo development rates were compared between the different experimental groups. Only
the number of cleaved oocytes was less when ICSI with no activation was used (p < 0.05).
cleavage and embryo development rates were compared between the different experimental groups. Only
the number of cleaved oocytes was less when ICSI with no activation was used (p < 0.05).
cleavage and embryo development rates were compared between the different experimental groups. Only
the number of cleaved oocytes was less when ICSI with no activation was used (p < 0.05).
◦C in a 25% (v/v) collagenase solution. The
cleavage and embryo development rates were compared between the different experimental groups. Only
the number of cleaved oocytes was less when ICSI with no activation was used (p < 0.05).p < 0.05).