Implication of RANTES in the modulation of alloinmune response by progesterone during pregnancy

RAMHORST R; GUTIERREZ G (COMPARTIENDO EL PRIMER LUGAR); CORIGLIANO A; JUNOVICH G; FAINBOIM L

Am J Reprod Immunol

Blackwell

Lugar: Oxford; Año: 2007 vol. 57 p. 147 - 152

1046-7408

Problem: Several studies indicate that RANTES is able to down-regulate T-cell responses which suggest it might be relevant for fetal-tolerance induction. However, the role of RANTES in pregnancy had not been established. Here we investigate RANTES regulation during early pregnancy and potencial failures leadding pregnancies losses. Method of Study: RANTES and progesterone levels were determined in sera and feto-placental units from high resorption rate CBA/J x DBA/2 pregnant females and compared to CBA/J x BALB/c normal pregnant mice. RANTES in vitro modulation was also studied in nulliparous, primiparous and multiparous CBA/J and BALB/c cells in response to paternal allo-antigen and progesterone stimulation. Results: Nulliparous CBA/J females were quantitatively deficient in RANTES sera levels, whereas pregnancies with male BALB/c or DBA/2 increased its production. However, feto-placental units from CBA/J females are higher producers of progesterone and RANTES. Conclusions: These data suggest that RANTES-beneficial effect on feto-maternal interface requires an optimal concentration range and might be modulated by progesterone, hence and exacerbated placental expression could be associated to high resorption rate. Problem: Several studies indicate that RANTES is able to down-regulate T-cell responses which suggest it might be relevant for fetal-tolerance induction. However, the role of RANTES in pregnancy had not been established. Here we investigate RANTES regulation during early pregnancy and potencial failures leadding pregnancies losses. Method of Study: RANTES and progesterone levels were determined in sera and feto-placental units from high resorption rate CBA/J x DBA/2 pregnant females and compared to CBA/J x BALB/c normal pregnant mice. RANTES in vitro modulation was also studied in nulliparous, primiparous and multiparous CBA/J and BALB/c cells in response to paternal allo-antigen and progesterone stimulation. Results: Nulliparous CBA/J females were quantitatively deficient in RANTES sera levels, whereas pregnancies with male BALB/c or DBA/2 increased its production. However, feto-placental units from CBA/J females are higher producers of progesterone and RANTES. Conclusions: These data suggest that RANTES-beneficial effect on feto-maternal interface requires an optimal concentration range and might be modulated by progesterone, hence and exacerbated placental expression could be associated to high resorption rate. Problem: Several studies indicate that RANTES is able to down-regulate T-cell responses which suggest it might be relevant for fetal-tolerance induction. However, the role of RANTES in pregnancy had not been established. Here we investigate RANTES regulation during early pregnancy and potencial failures leadding pregnancies losses. Method of Study: RANTES and progesterone levels were determined in sera and feto-placental units from high resorption rate CBA/J x DBA/2 pregnant females and compared to CBA/J x BALB/c normal pregnant mice. RANTES in vitro modulation was also studied in nulliparous, primiparous and multiparous CBA/J and BALB/c cells in response to paternal allo-antigen and progesterone stimulation. Results: Nulliparous CBA/J females were quantitatively deficient in RANTES sera levels, whereas pregnancies with male BALB/c or DBA/2 increased its production. However, feto-placental units from CBA/J females are higher producers of progesterone and RANTES. Conclusions: These data suggest that RANTES-beneficial effect on feto-maternal interface requires an optimal concentration range and might be modulated by progesterone, hence and exacerbated placental expression could be associated to high resorption rate. Problem: Several studies indicate that RANTES is able to down-regulate T-cell responses which suggest it might be relevant for fetal-tolerance induction. However, the role of RANTES in pregnancy had not been established. Here we investigate RANTES regulation during early pregnancy and potencial failures leadding pregnancies losses. Method of Study: RANTES and progesterone levels were determined in sera and feto-placental units from high resorption rate CBA/J x DBA/2 pregnant females and compared to CBA/J x BALB/c normal pregnant mice. RANTES in vitro modulation was also studied in nulliparous, primiparous and multiparous CBA/J and BALB/c cells in response to paternal allo-antigen and progesterone stimulation. Results: Nulliparous CBA/J females were quantitatively deficient in RANTES sera levels, whereas pregnancies with male BALB/c or DBA/2 increased its production. However, feto-placental units from CBA/J females are higher producers of progesterone and RANTES. Conclusions: These data suggest that RANTES-beneficial effect on feto-maternal interface requires an optimal concentration range and might be modulated by progesterone, hence and exacerbated placental expression could be associated to high resorption rate.