LONG-TERM EFFECTS ON HIPPOCAMPAL PROBDNF FOLLOWING SUCROSE CONSUMPTION IN JUVENILE VERSUS ADULT RATS.

KRUSE M.S.; COIRINI, H.; REY, M.

CABA

Congreso; IV REUNIÓN CONJUNTA DE SOCIEDADES DE BIOLOGÍA DE LA REPÚBLICA ARGENTINA; 2020

Sociedad Argentina de Biología

Excessive consumption of sucrose in early stages of development has deleterious neurobiological and behavioral effects in adulthood. We previously reported difficulties in memory retrieval. Here, we examined the proBDNF expression in the ventral hippocampus (vHIP) and media prefrontal cortex (mPFC) of animals exposed to sucrose during youth (SY) or adulthood (SA) by Western blot. Two-way ANOVA showed significant differences among groups in the vHIP (Fi(1,16)=13.456; p=0.003). Animals SY showed a decrease pBDNF levels (Fisher´s LSD post hoc test, p=0.035) while animals SA showed a raise of these values (Fisher´s LSD post hoc test, p= 0.013).When all animals were considered, the proBDNF levels correlated positively with the exploration ratio in two memory tasks T3 and T4 (one-way ANOVA, FT3(1,19)=5.470 p=0.0334; r2=0.268 and FT4(1,19)=14.617 p=0.0034; r2=0.3076) indicating that higher levels of proBDNF corresponds to better memory response. No differences in proBDNF levels were found in the mPFC (two-way ANOVA, Fi(1,16)=0.539; p=0.4743). Taken together, these results show that sucrose affects long-term BDNF expression in vHIP and these abnormalities are different depending on the age of exposure. In addition, it also demonstrates that animals exposed to unlimited consumption of sucrose during youth require higher levels of proBDNF to achieve the same memory response as a control animal in adulthood.